Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 10;42(16):e127. doi: 10.1093/nar/gku604

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Design of Rci cassettes. (A) The Rci cassette is formed by concatenation of n sequence fragments, in which individual fragments are separated by a single sfx site—the orientation of which alternates between successive fragments. Upon Rci recombination, the cassette is shuffled. Because Rci cannot mediate excision events, the cassette remains the same length, but the relative position of each fragment within the cassette can change. (B) sfx sites share a central spacer sequence and right arm sequence, but have little sequence conservation in the left arm. Five different sfx sequences were used in this study. (C) The diversity of the Rci cassette as a function of the number of fragments, n. Cassettes with >20 fragments can generate sufficient diversity to label all cells in complex organs/organisms. (D) The diversity as a function of length for Rci cassettes (assuming 100bp fragments). Lengths ∼1–2 kb are needed to reach the requisite diversity of 10⁹.