Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Activity of wild-type and mutant Dbr1 enzymes. (a) An in vivo complementation assay was used to assess debranching in Δdbr1 stains of the model organism S. cerevisiae. Northern blotting allows monitoring of accumulated lariat RNA when Dbr1 has been deleted or inactivated. Levels of U6 snRNA were used to normalize each strain tested. (b and c) In vitro debranching assay using recombinant wild-type and C14S Dbr1 and a synthetic substrate analog (46).
