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. 2014 Aug 28;42(16):10731–10747. doi: 10.1093/nar/gku612

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Growth of ΔRSA1, ΔHIT1, ΔPIH and ΔRSA1-ΔHIT1 BY4741 mutant cells. (A) Growth of WT and mutant strains in YPD medium was monitored over time by A₆₀₀ measuring. Doubling times (G) were calculated and growth defects are expressed as a percentage of the doubling time of the WT strain. (B) Strains lacking Rsa1p and Pih1p, or Hit1p and Pih1p are not viable. Diploid strains generated from crossbreeding between ΔRSA1, ΔPIH1 or ΔHIT1 haploid BY4741 (MATa) strains with BY4742 (MATα) strain were placed under starvation conditions for meiosis and spore formation. Tetrads were dissected and haploid spores were deposited on YPD plates and incubated for one week at 30°C. A representative dissection of tetrads is shown for each crossbreeding.