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. 2014 Aug 20;42(16):10579–10595. doi: 10.1093/nar/gku762

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Novel transcripts and sRNA. (A) Northern blot validation of putative small RNAs. Expression of three new transcripts and two previously published sRNAs could be confirmed in MS11 strain. Wt MS11 as well as mutant strains were analyzed by northern blotting. Calculated RNA length were: 115 nt (short transcript) and 191 nt (long transcript) for NgncR_198, 88 nt for NgncR_162, 75 nt for NgncR_011, 239 nt for NgncR_036 and 109 nt for NgncR_094. Multiple bands visible for NgncR_198, NgncR_036 and NgncR_094 may have originated from degradation of the full-size products. (B) Visualization of sequence reads using the integrated genome browser for putative small RNA NgncR_011 and NgncR_198 obtained from non-treated and TEX-treated (+ TEX) libraries.