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. 2014 Aug 20;42(16):10711–10719. doi: 10.1093/nar/gku768

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Proteomic analysis of BW25113 strains lacking efp, epmA, epmB and epmC. (A) Scatter and density plots of the inverted normalized H/L ratios (log2-transformed) relative to the summed-up protein intensity for a SILAC data from the Δefp, ΔyjeK, ΔyjeA and ΔyfcM for all proteins (grey) and PPP-containing proteins (orange). (B) Heat map representation of the 30 identified PPP-containing proteins that were up- (red) and down- (blue) regulated in the Δefp, ΔyjeK, ΔyjeA and ΔyfcM strains relative to wild-type strain. (C) In vitro translation time course of selected PPP-containing protein. Autoradiographs of SDS-PAGE and analysis of [³⁵S]Met-labelled LepA, Agp, NlpD, NudC, YcgL and ClsA. All reactions were performed in the absence (−) and presence (+) of active EF-P. The position of FL protein and intermediate peptidyl-tRNA indicated with black and blue arrows, respectively. Schematic representations of each protein are included, with the site of the PPP motif indicated by a blue arrow.