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. 2014 Aug 21;42(16):10321–10330. doi: 10.1093/nar/gku670

Figure 5.

Figure 5.

Effect of ATF4 RNAi on 5MP, ATF4 and GADD34 expression in T. casteneum. (A) and (B) RNA was isolated from adults 7 (panel A) or 20 days (panel B) after injection of 50 ng/μl (dashed lines) or 1 μg/μl (solid lines) of ATF4 dsRNA-477 or -478, ds RNA fragments corresponding to 5′- and 3′ half of ATF4 ORF, respectively. 5MP, ATF4, GADD34 and RPS3 mRNA levels were determined in triplicate in a single set of qRT-PCR (see Supplementary text) using gene-specific primers, as listed in Supplementary Table S1. The graphs show 5MP (top), ATF4 (middle) and GADD34 (bottom) mRNA levels relative to mock-treated control, after normalization by RPS3 data. Bars indicate SEM of the three technical replicates. (C) Correlation between GADD34 and ATF4 expression from data in (A) and (B).