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. 2014 Aug 21;42(16):10321–10330. doi: 10.1093/nar/gku670

Figure 6.

Figure 6.

Effect of 5MP RNAi on expression of 5MP, ATF4 and GADD34 in T. castaneum. (A) Relative mRNA expression for 5MP (top), ATF4 (middle), and GADD34 (bottom) in beetles 8 (panel 1) and 19 (panel 2) days post-injection (p. i.) of two non-overlapping RNAi fragments (1 μg/μl) directed to 5MP, termed 5MP dsRNA-616 or dsRNA-690. RNA abundance was quantified with qRT-PCR and presented as in Figure 5A and B. (B) Expression in eggs 0–12 h (panel 1) or 12–24 h (panel 2) post-lay (p. l.) by injected adults. In panel 1, 5MP, ATF4 and GADD34 expression normalized to RPS3 was examined right after the injected adults were allowed to lay eggs for 12 h and presented in values relative to expression from mock-treated adults. In panel 2, expression was similarly examined after the eggs collected similarly were aged for an additional 12 h. (C) 5MP, ATF4 and GADD34 expression normalized to RPS3 in larvae 3 days post-lay by injected adults. Presented are values relative to expression from mock-treated adults. In (B) and (C), the average of two biological replicates are shown with SEM.