Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 13;42(16):10516–10528. doi: 10.1093/nar/gku746

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Human 9-1-1 complex stimulates DNA2 and EXO1 activities. (A, B) DNA2 cleavage activities on a fork (A) and a flap (B) substrate in the presence of the 9-1-1 complex or PCNA. Labeling of the substrate at the 3′ end allows the determination of the final products of the reaction. Products are analyzed on alkaline gels as described in the Materials and Methods section. (C, D) EXO1 cleavage activities on a 3′overhang (C) and a flap (D) structure in the presence of 9-1-1 or PCNA. Substrates used for each panel of the experiment are depicted on the top of the gel with the asterisk indicating the site of the ³²P label. The flap is 30 nt long. The labeled strand in panel (C) is 60 nt long.