. 2014 Aug 26;42(16):10655–10667. doi: 10.1093/nar/gku774

Table 2.

Calculated observed rates of PCNA-AF488₂ closing with RFC

DNA substrate	−RPA		+RPA
	k_obs1 (s⁻¹)^a	k_obs2 (s⁻¹)	k_obs1 (s⁻¹)^a	k_obs2 (s⁻¹)
3′DNA	3.3 ± 0.2 (2.5)	0.44 ± 0.01	4.4 ± 0.5 (49)	0.33 ± 0.07
3′DNA, no 5′P	2.9 ± 0.3 (2.2)	0.44 ± 0.07	4.6 ± 0.7 (51)	0.26 ± 0.02
5′DNA	1.3 ± 0.1 (1)	0.27 ± 0.03	0.09 ± 0.01 (1)	N/A
5′DNA, no 5′P	1.2 ± 0.1 (0.9)	0.29 ± 0.03	0.79 ± 0.04 (8.8)	0.35 ± 0.02

^aRates relative to the rate of 5′DNA are given in parentheses to show the fold change.

N/A indicates no observed rate. Observed rates are calculated from Equations (2) and (3) (5′DNA·RPA only) and represent the average and standard deviation of three independent experiments.