Table 3.

Calculated observed rates of β-AF488₂ closing with ‘χ-less’ γ complex (γ₃δδ′ψ)

DNA structure	−SSB	+SSB
	kobs (s−1)a	kobs1 (s−1)a
3′DNA	5.2 ± 0.2 (3.0)	1.8 ± 0.3 (30)
3′DNA, no 5′P	6.1 ± 0.4 (3.6)	2.7 ± 0.1 (45)
5′DNA	1.7 ± 0.1 (1)	0.06 ± 0.02 (1)
5′DNA, no 5′P	1.8 ± 0.2 (1.1)	1.0 ± 0.2 (17)

^aRates relative to the rate of 5′DNA are given in parentheses to show the fold change.

Observed rates are calculated from Equation (1) and represent the average and standard deviation of three independent experiments.