Figure 5.

Effects of DNA sequences on internal CSL dynamics and functional implications. (A–E) Results from MD simulations showing inter-domain correlations when CSL is bound to different sequences as indicated. In particular, A–C show the comparison between the projection of the first eigenvector on the CSL structure, in the presence or absence of DNA sequences. The spikes of the porcupine plots indicate the principal motions (i.e. the motion described by the first eigenvector) for each C-alpha, while the length indicates the intensity of the motion. A’–C’ and D–E show a comparison of the ICRM matrices between the different systems studied. (F) Consequences of mutations in CSL on activity of reporter genes containing the indicated sequences. Unmutated CSL (WT), single mutant CSL (415A) and triple mutant CSL (413AAA) were co-transfected with NICD (ratio of DNAs was 1:5) and the expression of the indicated reporter measured relative to co-transfected renilla control. Error bars depict the standard error of the mean from >3 biological replicates. (G) Adult wings from flies with either wild-type or mutated CSL rescue plasmids, right panel is a higher magnification of the region with altered venation (blue circles). Tables indicate the proportion of flies that were viable in each case, and the proportion of viable flies whose wings had venation defects. N > 200.