Table 2.
Calorimetric data for various DNA sequences binding to CSL
| Ligand (syringe) | K (M−1) | Kd (μM) | ΔG° (kcal/mol) | ΔH° (kcal/mol) | −TΔS° (kcal/mol) |
|---|---|---|---|---|---|
| CGTGGGAA | 2.2 ± 1.4×107 | 0.06 | −9.4 ± 0.3 | 5.1 ± 1.0 | −14.5 ± 0.7 |
| TGTGGGAA | 2.0 ± 0.5×106 | 0.50 | −8.2 ± 0.1 | 8.8 ± 1.2 | −17.0 ± 1.0 |
| CGTGTGAC | 5.9 ± 1.0×105 | 1.72 | −7.5 ± 0.1 | 4.8 ± 1.0 | −12.3 ± 1.0 |
| CGTAAGAA | — | >50 | — | — | — |
| TCATACCT* | NBD | NBD | NBD | NBD | NBD |
*Negative control.
All experiments were performed at 10°C. Values are the mean of at least three independent experiments and errors represent the standard deviations of multiple experiments.
NBD = no binding detected.