Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 27;20:1411–1421.

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

PMC Copyright notice

Induction of pigment epithelium derived factor (PEDF) and suppression of exacerbating factors at mRNA levels by 5-aza-2’-deoxycytidine (5-aza-dC) in human retinal endothelial cells (HRECs) and human retinal pigment epithelial (HRPE) cells in normal glucose (NG) condition. Similar effects of trichostatin A (TSA) was observed except that intercellular cell adhesion molecule-1 (ICAM-1) mRNA was induced in HRECs by TSA. Cells were cultured for 48 hours in NG with or without 5-aza-dC (5 μM, 10 μM) or TSA (0.2 μM, 0.5 μM, 1 μM). PEDF, vascular endothelial growth factor (VEGF), intercellular cell adhesion molecule-1 (ICAM-1), interleukin (IL)-1β, and matrix metalloproteinase 2 (MMP2) gene expressions was analyzed with real-time PCR. The data are expressed as the mean ± standard deviation (SD; *p<0.05). All experiments were repeated three times with similar results.