Figure 2.

Oryzalin Partially Rescues the ark1-1 Root Hair Morphology.

(A) Bright-field images of wild-type and ark1-1 root hairs treated with either 5 μM taxol or oryzalin. The root hair phenotype of ark1-1 is partially rescued with oryzalin but not with taxol. Bars = 50 μm.

(B) Mean length of wild-type and ark1-1 root hairs treated with various concentrations of taxol and oryzalin. ark1-1 exposed to 100 nM, 1 μM, and 5 μM oryzalin partially rescues the root hair length phenotype. Data and bars represent mean and sd, respectively. Asterisks represent a significant increase in mean length relative to untreated ark1-1 root hairs. A minimum of 25 root hairs were measured for each genotype and treatment.

(C) Frequency distribution histograms comparing root hair morphology types in the wild type and ark1-1 grown on varying oryzalin concentrations. Oryzalin (1 and 5 μM) partially mitigates the ark1-1 root hair defect by shifting the distribution of the most severe branched phenotype to a milder wavy/bulbous phenotype. As a control, wild-type root hairs show an increase loss of root hair polarity upon exposure to increasing oryzalin concentrations. A minimum of 10 roots and 150 root hairs were measured for each treatment.