Table 2.
Estimated Proportion of Wild-Type (wt; I221) Neuraminidase (NA) in Clinical Specimens, Compared With the Oseltamivir Phenotype for the Corresponding Virus Isolate
| Days After Clinical Onset | Type of Specimen | NA Gene Level, Copies/mL Specimena | wt (I221) NA Proportion, % | Virus Recovered | Oseltamivir Phenotype |
|---|---|---|---|---|---|
| 12 | NPA | 280 000 | 100 | B/Lyon/CHU/12.88/2011 | NI (wt) |
| 18 | NPA | 4 760 000 | 100 | B/Lyon/CHU/12.1127/2011 | NI (wt) |
| 22 | NPA | 6 090 000 | 100 | B/Lyon/CHU/13.584/2011 | NI (wt) |
| 28 | NPA | 217 000 | 100 | B/Lyon/CHU/14.411/2011 | NI (wt) |
| 30 | NPA | 770 000 | 100 | B/Lyon/CHU/14.767/2011 | NI (wt) |
| 34 | NPA | 4 172 000 | 1 | B/Lyon/CHU/15.216/2011 | HRI (221L) |
| 35 | NPA | 6300 | 100 | B/Lyon/CHU/15.489/2011 | NI (wt) |
| 37 | NPA | 3 920 000 | 11 | B/Lyon/CHU/15.731/2011 | NI (wt) |
| 40 | N | 259 000 | 5 | B/Lyon/CHU/16.167/2011 | HRI (221L) |
| 42 | BAL | 4 018 000 | 0.70 | B/Lyon/CHU/16.432/2011 | HRI (221L) |
Quantification of the wt or mutant NA was performed using real-time reverse-transcription quantitative polymerase chain reaction, using specific probes for I221 (wt) or 221L (mutant) NA, as described in Materials and Methods.
Abbreviations: BAL, bronchoalveolar lavage; N, nasal swab; NPA, nasopharyngeal aspirate.
a Data total no. of I221 (wt) and 221L (mutant) NA gene copies.