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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Parasitol Res. 2014 Jul 17;113(10):3685–3696. doi: 10.1007/s00436-014-4033-3

Fig. 8.

Fig. 8

Inhibition of glycocalyx shedding enhanced MAC formation on the cercarial surface. Cercariae were treated for 2h with normal human serum in the presence of eserine sulfate and stained with anti-neoC9. Parasites had assorted patterns of fluorescence (a–d) due to partial glycocalyx shedding. There is no staining at the anterior tip of the parasites (a2, arrow) due to inhibition of acetabular gland secretion by eserine. Culturing for additional 16 h in medium without eserine promoted complete shedding of the glycocalyx seen as large particles in the bottom of the plate (e1 and e2, arrows). Scale bars are 100 μm in a 20× magnification field