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. 2014 Jul 4;3(2):339–352. doi: 10.1016/j.stemcr.2014.06.001

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

Nonoverlapping Expression of Different Tissue-Associated Genes in X1 Neoblasts

Cell FISH from sorted X1 cells from prepharyngeal regions 48 hr following amputations using several combinations of genes expressed in different tissues. Pharynx: FoxA; CNS: pax6A, neuroD-1, and sp6-9; eye: ovo and sp6-9; protonephridia: POU2/3, sall, osr; muscle: collagen; anterior pole: FoxD, notum, prep, and gut: hnf4 and gata4/5/6. No overlapping expression within the same X1 cells was detected (paired t test one-tailed, p = 0.0064) when compared to expected frequencies. Total number of X1 cells counted: FoxA/pax6A n = 693, FoxA/ovo n = 198, POU2/3/sall/osr/pax6A n = 489, FoxD/prep/notum/pax6A n = 255, collagen/sp6-9 n = 198, collagen/FoxA n = 443, FoxA/POU2/3/sall/osr n = 295, hnf4/sp6-9 n = 448, collagen/POU2/3/sall/osr n = 427, hnf4/pax6A n = 216, POU2/3/sall/osr/gata4/5/6 n = 2422, neuroD-1/gata4/5/6 n = 201. DAPI labeled DNA (gray). Images shown are maximal-intensity projections. Scale bars, 10 μm.