Figure 4.

Origin accessibility is increased in the absence of Rpd3. Aggregated nucleosomal profiles of 318 NDR associated with replication origins (A) or 320 NDR non-associated with origins (B) from G1-arrested WT (blue) and rpd3Δ (red) cells were aligned relative to the ACS or to the NDR center, respectively. (C), Average NDR width of replication origins (left graph) or non-origin regions (right graph) from WT (blue) and rpd3Δ mutants (red). (D), As in A but for early origins. (E), As in A but for late origins. (F), As in C but for early (left graph) and late origins (right graph). (G), Nucleosome patterns around the late origins ARS 603 and ARS 423 from WT (blue) and rpd3Δ samples (red). Hyper-acetylated regions in the rpd3 mutant (Robyr et al., [31]; Vogelauer et al. [9]) are represented in green. Black arrows point to nucleosomes affected in the absence of Rpd3.