Fig. 5.

Effect of resveratrol and methylthiostilbenes on Stat3 activation in HaCaT (a) and A431 (b) cells. Activated Stat3 was assessed in terms of the amount of Stat3 contained in the DNA-binding complexes extracted from the nuclei that were isolated from the cells. The Stat consensus site (5′-TTCCCGGAA-3′) was immobilized on ELISA plates as bait. Bars represent mean ± SEM from three independent experiments run in duplicate. The asterisk above the bar denotes statistically significant differences from the control cells (DMSO), p < 0.05