Table 1.
Primers for gene expression validation with qPCR and amh PCR and sequence analysis
| Gene symbol/probe | Description | Accession number | Forward and reverse primers (from 5’ to 3’) |
|---|---|---|---|
| tspan8 | tetraspanin-8 | XM_003448079 | TGTATGTTGTGGAATAGGCATCA |
| GGTGATTTGTAAAGCTGTTTCG | |||
| fcgrt | major histocompatibility complex class I-related gene protein-like | ENSONIG00000008406 | TGGTGTGAGCAAGGACTTCAT |
| ACATGAACCAAAGGACTGTAAACT | |||
| cr/20β-hsd | carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase | XM_005473633 | CCAAAATTGTTCGTTTTATTCTCG |
| TTTCATTTTGATGCGTTCCA | |||
| gpa33 | cell surface A33 antigen | XM_005466820 | AATGTCCAAAAGCCAACCTAAA |
| TACTACATCTGCACCTCGGAGA | |||
| rtn4Ip11 | reticulon-4-interacting protein 1 homolog, mitochondrial | XM_003459954 | GCATGTCAAGGCATCAAATAAA |
| CCTCTGCTGGTTGTAAATGTGA | |||
| 26098 | hypothetical protein | TTCCTGAAGACAGTACAGTACAAAA | |
| CGAATTCTTCTGGTCAAGTTCTTC | |||
| zp3 | zona pellucida sperm-binding protein 3 receptor-like | XM_005448435 | TGTCTGTAACTACTCATTTGGATCA |
| CCATTTTACAGATCCAACTTTCC | |||
| gapdh | glyceraldehyde-3-phosphate dehydrogenase | XM_003452690 | GGCATCGTGGAAGGTCTCAT |
| CATTTTACCAGAGGGCCCGT | |||
| rpp30 | ribonuclease P protein subunit p30 | XM_005471600 | CCCGACTCCTATCAACGAAC |
| AAAGTGACTCGCGTCTGACA | |||
| amh | anti-Müllerian hormone1 | DQ257619.1 | TTCTTATCGCTCCGACTTCTTC |
| TAGGGCTGGTTGATATGGAATC | |||
| anti-Müllerian hormone - exon VII2 | XM_003451305 | AGCAGCTCTAGCGGCATCCACA | |
| TGTGTTTTCTTTCTGCGTCCGCCA | |||
| 13: 5’ UTR, exon I, intron 1, exon II | ENSONIG00000004781 | AGAGGAGTCATCAGTCCAAAGC | |
| AGATGTCCTCCACGAAGCAT | |||
| 2: exon II, intron II, exon III | ENSONIG00000004781 | AAGACCCCATCATCACCATC | |
| TTGTCTGAGCCGTAATCTGC | |||
| 3: exon IV, intron III, exon V, intron IV, exon VI | ENSONIG00000004781 | GGAAAATCATCAGAGGGGAGT | |
| CTGCCGACTTCAGAACTTTT | |||
| 4: intron VI, exon VII, 3’ UTR | ENSONIG00000004781 | CGGTCCCAGTGACCTATGAG | |
| AAGTACACGTGGTGTATTGTAATTGA | |||
| 5: 3’ UTR | ENSONIG00000004781 | CCCCAGCATTTATAACTTTCACA | |
| CCTGCCTCAAGTATGCCTTT | |||
| LM4: intron VI, exon VII | ENSONIG00000004781 | TGTGTTTTCTTTCTGCGTCCGCCA | |
| AGCAGCTCTAGCGGCATCCACA |
1qPCR validation of microarray.
2PCR of cDNA.
3set of 5 primer pairs for sequencing.
4Linkage mapping.