Table 2.

Gene expression validation of microarray results

				qPCR
Over expressed in	Gene symbol	Microarray		cDNA		Multiple Tm peaks3	gDNA
		FC1	p-value	FC	p-value		FC	p-value
Male	amh	0.5	6 × 10−9	0.27	0.002		0.75	7.4 × 10−4
	tspan8	0.2	4.4 × 10−16	0.0001	5 × 10−11		0.0013	1.2 × 10−4
	fcgrt	0.5	6.5 × 10−8	ND2		√	ND
Female	cr/20β-hsd	3.5	1.0 × 10−17	352	2.8 × 10−11	√	669	0.002
	gpa33	3.4	6.0 × 10−9	88.7	2.6 × 10−5	√	15.8	3.3 × 10−6
	rtn4Ip1	2.5	1.0 × 10−17	2.1	0.0001		ND
	CUST_26098	4.2	1.6 × 10−7	4.46	0.004		ND
	zp3	2.6	3.0 × 10−9	2	0.004		ND

Mean ratio of female to male gene expression by qPCR from cDNA of embryos from 2 to 9 dpf is presented. The qPCR analysis of genomic DNA (gDNA) was applied for indication of copy number variation.

¹FC: fold change of female to male expression: LS means for Microarray and means for qPCR based on cDNA of embryos from 2 to 9 dpf.

²ND: not determined.

³Multiple TM peaks resulting from melting curve analysis.