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. 2014 Aug 28;15(1):734. doi: 10.1186/1471-2164-15-734

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© Mazzon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Localization of Zur binding sequences in the regulatory regions of Zur-regulated genes. (A) The putative Zur-binding sites predicted in silico are shaded. Arrows indicate transcriptional start sites experimentally identified in [39] which allowed the prediction of −35 and −10 promoter elements. Asterisks indicate base changes through site-directed mutagenesis in the putative Zur-binding site at the promoter region of the CC2720-26 operon. (B) Alignment of nucleotide sequences containing the putative Zur binding site in the promoter of Zur-regulated genes. Bold letters and arrows indicate inverted repeats sequences used in in silico screening for putative Zur-regulated genes. (C) DNA sequence logo representing the C. crescentus Zur-binding site was obtained by using the WebLogo generator.