Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Neurotoxicology. 2014 Jun 10;44:80–90. doi: 10.1016/j.neuro.2014.06.001

Figure 7. 4R injected 1 hour before DFP decreased neuronal death and astrocyte activation.

Figure 7

Micrographs (10X) are from a 4R vehicle control (1hDMSO-PI-DFP) and a rat preinjected with 4R (1h4R-PI-DFP). The magnification of the insets for Fluoro-Jade B was 30X and for amino cupric silver and nestin, 40X. (A) Fluoro-Jade B staining. The number of Fluoro-Jade B stained cells in the stratum pyramidale was significantly lower in the 4R group than in the 4R vehicle control (n =10 rats per group). (B) Amino cupric silver staining. The number of amino cupric silver-stained cells in the stratum pyramidale was significantly lower in the 4R group than in the 4R vehicle controls (n=10 per group). (C) Immunostaining for nestin. The number of activated astrocytes in the stratum radiatum was significantly lower in the 4R group than the 4R vehicle control which was injected with DMSO (n= 6 rats per group). The bars are means ± SEM. See statistics in Methods. See Fig. 2C for experimental design.