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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Curr Opin Struct Biol. 2014 Mar 25;0:1–7. doi: 10.1016/j.sbi.2014.02.005

Figure 2. Cryo-EM data are significantly improved with direct detection camera and motion correction.

Figure 2

(a) Fourier transform of a typical cryo-EM image of TRPV1 ion channel embedded in thin layer of vitreous ice recorded with the direct detection camera, K2 Summit, after motion correction. Thon rings are visible up to ~3Å resolution. The position corresponding to 8Å is also marked. (b) Fourier transform of a similar image recorded using a phosphor scintillator based CMOS camera, TemF816. Thon rings are visible up to ~8Å resolution. (c) Three typical 2D class averages of images recorded with the K2 camera. (d) Similar 2D class averages of TRPV1 particle images recorded with the TemF816.