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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Curr Opin Struct Biol. 2014 Mar 25;0:1–7. doi: 10.1016/j.sbi.2014.02.005

Figure 3. 3D classification of particle images of the TRPV1 ion channel.

Figure 3

(a) A total of 45,625 particle images were classified into six classes. 3D reconstructions calculated for each class are shown. The number of particles in each class is: 10,357 (I); 9,527 (II); 3,778 (III); 11,671 (IV); 5,328 (V); 4,964 (VI). Only the first class can be refined to high resolution, 8.8Å. (b) Two different views of the 3D reconstruction calculated from the entire dataset. The nominal resolution is ~9.3Å, but the center of the transmembrane domain is hollow, showing no sign of transmembrane helices. (c) The final 3D reconstruction calculated from class I at a nominal resolution of 8.8Å, showing all transmembrane helices.