Figure 3. Clinically relevant concentrations of paclitaxel cause cell death.
(A) Low nM concentrations of paclitaxel cause a decrease in live cell number over 120 hours in MDA-MB-231 (left) and Cal51 (right) cells. (B) Trypan blue assay showing an increase in the proportion of dead cells in both MDA-MB-231 (left) and Cal51 (right) cell lines by 120 hours of paclitaxel treatment. (C) Low nM paclitaxel inhibits the ability of cells to form colonies after 14 days of paclitaxel treatment. No colony formation was observed at concentrations above 100 nM paclitaxel in either cell line. (A–C) n ≥ 3 independent experiments. * = p < 0.05 as compared to DMSO control by Wilcoxon (A, C) or Mantel-Haenszel (B) statistical analysis. Exact p values are listed in Table S2.