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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: J Allergy Clin Immunol. 2013 May 31;132(1):170–181. doi: 10.1016/j.jaci.2013.04.048

Fig. 1. High dose Ag tolerization of the CS immune response induces suppressor T cells (Ts) producing suppressive supernatant (Ts Sup) containing exosome-like nanovesicles.

Fig. 1

a. Ts cells (Group B) or their culture supernatant (Ts Sup, Group C) suppressed adoptive transfer of CS. b. Electron microscopy revealed that Nl Sup, Sham Sup and TNP-Ts Sup pellets contained nanovesicles resembling exosomes at 80,000 times magnification (the bar is 65nm). c. Nanoparticle tracking analysis (NTA) showed homogenous sized nanovesicles from control RAW cell line supernatant and from TNP-Ts Sup pellets, indicating particle size/concentration. d. Western immunoblotting showed CD9 tetraspanin expression by extracts of pellets from TNP-Ts Sup, Nl Cell Sup, Sham Sup and RAW cell line Sup, compared to control RAW cell lysate.