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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: J Allergy Clin Immunol. 2013 May 31;132(1):170–181. doi: 10.1016/j.jaci.2013.04.048

Fig 3. Determination that the Ts Sup suppressive exosome-like nanovesicles are derived from CD8+ cells, are present in plasma of Ts donors, and are not produced by Treg cells.

Fig 3

a. Treatment of Ts cells from tolerized mice with anti-CD8 mAb + C’ prior to culture to derive Ts Sup eliminated suppression of adoptivelly transferred CS (C). Similar anti-CD4 mAb treatment of the OX-Ts had no effect (D). b. Tolerized Ts donor plasma nanovesicles were suppressive (E), whereas nanovesicles from other sources (C, D, F, G) were non-inhibitory. c. Only TNP-Ts Sup and Ts donor plasma exosome-like nanovesicles inhibited the in vitro HT-2 cell response to IL-2 (B & D). d. DEREG mice depleted of Treg and wild type mice were tolerized with high Ag dose and showed similar suppressive ability (C & D).