a. Dual Reciprocal Ag-Specificity of Ts Sup Exosome-like nanovesicles. TNP-CS effector cells only, positive control (A), suppression in the TNP homologous system (B), no suppression in the TNP heterologous system (C), OX-CS effector cells only, positive control (D), no suppression in the OX heterologous system (E), suppression in the OX homologous system (F). b. Flow cytometry analysis of kappa light chain expression on TNP Ts Sup (right, red) and RAW cell-derived (left, blue) nanovesicles. Isotype controls are shown in gray. c. Nanovesicles from TNP-tolerized JH-/- mice did not mediate suppression (C). In vitro addition of monoclonal anti-TNP Ab LC to nanovesicles from tolerized JH-/- reconstituted suppression (D). d. The Non-Ag-specific assay of HT-2 T cell responsiveness to IL-2 showed that nanovesicles from tolerized JH-/- mice had strong non-Ag specific suppressive activity (Group C vs. A), equivalent to that of wild type Ts Sup nanovesicles (B vs. A).