Figure 5.

ViLVaL combination therapy contributes to maintenance of islet morphology and increases the insulin content of the pancreas. Insulin content was measured in pancreatic extracts (A). The proportion of beta-cells to the whole pancreas was calculated using insulin-stained pancreatic sections (B). Sections of pancreas were immunostained for insulin with glucagon (C to G), PDX-1 (H to L), IRS-2 (with DAPI; M to Q), NF-κB p65 subunits (R to U) or HSP72 (V to Z). Scale bars indicate 20 μm at × 400 magnification. Values represent mean ± SEM, n = 6 – 8. *p < 0.05, **p < 0.01 compared with the CT group. †p < 0.05 compared with the ViL group.