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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Exp Dermatol. 2014 Oct;23(0 1):2–6. doi: 10.1111/exd.12389

Figure 4.

Figure 4

UV irradiation increases protein binding to inverted CCAAT box in the TβRII proximal promoter in human skin fibroblasts. A) Sequence of TβRII promoter spanning nucleotides −100 to −62 used as probes for EMSAs. Wild-type and mutant inverted CCAAT sites are bolded and underlined, respectively. B) EMSAs were performed using [32P] TβRII wild-type or mutant probes. Nuclear extracts were prepared at indicated times after UV irradiation (50mJ/cm2). Closed triangle indicates specific retarded complex. Open triangle indicate nonspecific bands. C) Competition of protein binding to TβRII wild-type probe by 50-fold molar excess unlabeled wild-type or mutant probe. Nuclear extracts were prepared six hours after UV irradiation. Closed triangle indicate specific retarded complex. Results are representative of three experiments.