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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Exp Dermatol. 2014 Oct;23(0 1):2–6. doi: 10.1111/exd.12389

Figure 5.

Figure 5

NFY binds to the inverted CCAAT box in the TβRII promoter in human skin fibroblasts. Fibroblasts were mock or UV-irradiated (50mJ/cm2) and nuclear extracts were prepared six hours after irradiation. A) Competition of protein binding to TβRII promoter probe (spanning from −100 to −62) by 50-fold molar excess of unlabeled wild-type or mutant NFY probe. B) EMSA with [32P] TβRII promoter probe (spanning from −100 to −62) super shifted with control IgG or anti-NFYA antibody. Closed triangle indicates retarded complex; open triangle indicates super shifted complex. C) EMSA with [32P] NFY consensus probe super shifted with control IgG or anti-NFY-A antibody. Closed triangle indicates retarded complex; open triangle indicates super shifted complex. Results are representative of three experiments.