Table 2.

Regulation of transcripts of specific interest in the hypercapnia and elevated temperature experiments on Hyas araneus

Accession no.	Description	Rank	Treatment (I)	Treatment (II)	Treatment (III)	Treatment (IV)	Treatment (V)
HAAI01016321	uricase	10	−5.24	−4.27	−3.68	−7.10	−6.20
HAAI01006676	trehalose-6-phosphate synthase 1a	21	−4.45	−2.42	−2.13	−6.05	−4.57
HAAI01003297	cuticle proprotein	24	5.88	1.22	2.81	3.98	1.87
HAAI01001762	actin	53	−1.65	0.48	5.06	−0.34	−1.03
HAAI01004150	trehalose 6-phosphate synthase 1	61	−4.35	−1.68	−2.73	−4.88	−3.99
HAAI01015640	vitellogenin like	66	4.82	3.35	0.68	3.28	−0.13
HAAI01018061	peroxiredoxin	79	4.67	4.31	1.81	3.92	4.09
HAAI01010911	enoyl COA hydratase	80	−1.11	−0.65	0.34	−0.47	−4.67
HAAI01016834	like adducin related protein	83	4.62	3.22	0.81	3.30	0.29
HAAI01016838	GSH peroxidase like	87	3.67	4.61	2.81	3.92	4.19
HAAI01015788	vitellogenin like	100	4.48	3.61	1.22	3.60	−0.71
HAAI01000380	glucose-6-phosphat dehydrogenase	105	−3.16	−4.45	−2.56	−3.41	−2.87
HAAI01017747	sodium glucose cotransporter	113	2.94	4.36	0.64	1.98	1.29
HAAI01002706	trehalose 6-phosphate synthase 1b	117	−3.87	−1.10	−2.20	−4.32	−3.11
HAAI01012389	isocitrate dehydrogenase I	128	−1.18	−2.36	−0.55	−4.19	−2.88
HAAI01000602	dopa decarboxylase	138	−3.37	−2.78	−2.26	−2.80	−4.09
HAAI01003033	troponin I	175	−0.33	1.22	3.78	−0.02	0.29
HAAI01015542	heat shock protein 90	192	−3.07	1.07	−3.68	−0.09	−3.30
HAAI01002164	alpha carbonic anhydrase	197	3.67	1.80	1.64	3.45	1.87
HAAI01019079	ascorbate peroxidase	198	3.67	2.11	1.17	2.23	−2.52
HAAI01009105	heat shock protein 90	203	3.61	2.42	0.36	1.98	−2.03
HAAI01019113	vitellogenin like	226	3.52	2.39	−0.36	2.48	−1.56
HAAI01018669	vitellogenin like	234	3.50	2.29	0.15	2.00	−2.03
HAAI01016527	cytochrome p450 like	236	3.23	2.03	0.07	3.47	2.61
HAAI01009026	cuticle protein like	291	−0.47	3.20	0.01	0.98	−0.36
HAAI01010727	gelsolin precursor	303	1.67	0.68	3.13	0.64	1.29
HAAI01018844	alpha tubulin	309	3.11	1.41	1.46	2.74	−0.20
HAAI01008700	arginine kinase	338	3.01	2.27	1.43	2.76	2.14
HAAI01019135	carbohydrate phosphorylase like	344	2.67	1.48	1.26	2.99	1.21
HAAI01004058	cuticle protein	359	2.93	0.22	−0.55	0.20	−0.71
HAAI01015598	heat shock protein 90	367	2.90	1.47	−0.19	1.85	−0.71
HAAI01019120	bestrophin like	368	2.89	1.8	2.13	1.56	1.16
HAAI01000761	heat shock protein 70	381	2.84	1.94	1.30	2.61	1.37
HAAI01005842	cuticle protein like	386	2.81	0.48	1.54	0.63	−0.45
HAAI01001265	beta tubulin	403	2.77	1.77	0.97	1.98	1.02
HAAI01018645	V1-ATPase subunit	427	2.69	1.80	1.27	2.54	1.46
HAAI01018783	cuticle protein	435	2.67	0.71	1.49	1.50	1.61
HAAI01004930	heat shock protein 90	447	2.63	1.86	1.24	2.58	0.92
HAAI01007246	vitellogenin like	455	2.61	2.15	−1.10	0.98	−1.45
HAAI01003327	actin	461	2.53	2.26	1.06	2.60	1.72
HAAI01000796	alpha tubulin	466	2.59	2.06	1.09	2.34	1.70
HAAI01018213	alpha-glucosidase	477	1.83	1.26	1.56	2.55	2.39
HAAI01005237	alpha carbonic anhydrase like	478	2.55	1.80	0.52	2.42	0.91
HAAI01001455	cuticle protein like	498	−1.26	−1.86	−0.58	−2.44	−2.51
HAAI01014269	cuticle protein like	514	−0.50	−1.08	−0.57	−1.66	−2.48
HAAI01008420	troponin I	519	0.02	−0.73	−2.46	−0.88	−1.81
HAAI01002591	superoxide dismutase	535	−0.10	−1.65	−0.62	−1.49	−2.42
HAAI01019124	na + k + −atpase alpha subunit	536	0.81	0.22	1.82	2.09	2.41
HAAI01007529	glyceraldehyde 3-phosphate dehydrogenase	546	2.39	1.60	0.69	2.36	1.07
HAAI01004651	actin	556	2.38	1.44	0.52	1.82	1.33
HAAI01005807	cytochrome c oxidase subunit ii	559	2.23	1.82	0.96	2.37	1.33
HAAI01001460	cuticle protein like	560	−0.72	−1.31	−0.72	−1.62	−2.37
HAAI01001217	cuticle protein	562	2.37	1.10	2.21	0.40	−0.98
HAAI01003904	ankyrin related protein like	576	1.33	1.27	2.33	0.82	0.59
HAAI01000424	cytochrome c oxidase subunit i	583	2.32	1.56	1.10	2.28	0.84
HAAI01001438	nadh dehydrogenase subunit	598	1.95	1.46	0.87	2.27	1.29
HAAI01006730	heat shock protein 90	608	2.24	1.44	0.96	2.13	1.29
HAAI01005819	cuticle protein like	614	1.55	1.87	0.79	2.14	2.23
HAAI01015787	alpha tubulin	630	2.20	0.85	0.47	1.88	−0.27
HAAI01002070	vitellogenin like	634	2.20	1.77	−1.05	1.32	−1.57
HAAI01014788	thioredoxin	647	−1.73	−2.18	−0.50	−1.38	−1.52
HAAI01006091	gelsolin precursor	670	0.84	0.73	2.10	0.37	−0.28
HAAI01000874	alpha tubulin	673	2.03	2.09	0.90	1.31	1.69
HAAI01000485	cytochrome c oxidase subunit iii	711	1.94	1.28	0.60	2.02	0.90
HAAI01008219	gelsolin precursor	738	0.89	0.76	1.97	0.25	−0.35
HAAI01014184	thioredoxin	751	−1.08	−0.93	−1.23	−1.94	−1.80
HAAI01002593	thioredoxin peroxidase	769	−1.89	−0.72	−0.98	−1.52	−1.52
HAAI01005614	cuticle protein like	804	1.44	0.78	1.79	1.18	−0.21
HAAI01006347	cuticle protein like	817	1.28	−0.22	1.75	1.37	1.42
HAAI01018927	spectrin like	822	1.58	0.51	1.74	1.09	0.50
HAAI01000352	nesprin like	827	1.70	0.72	1.73	1.42	1.58

Transcripts significantly regulated in response to hypercapnia and elevated temperature as identified by DESeq analysis (for details, see Methods). Accession number (accession no.) refers to the transcriptome of Hyas araneus [40] and the database ENA (EMBL). Details on transcript description and transcript length are listed for each transcript. Transcripts are sorted according to the rank in absolute regulation regardless of the treatment. Changes are given in log₂-fold change for each treatment separately. Bold numbers represent significantly up-regulated transcripts; bold and italic numbers represent significantly down-regulated transcripts.