Figure 2.

Effects on ER-retained msGFP constructs of including the α-factor pro region after the α-factor signal sequence. S. cerevisiae cells carrying erv29Δ and htm1Δ alleles and expressing nuclear-targeted DsRed-Express2 were engineered to express msGFP with a C-terminal HDEL signal, fused to either the α-factor signal sequence alone (pre-αf-msGFP*-HDEL) or the complete α-factor secretion signal (pre-pro-αf-msGFP*-HDEL). Strains were imaged by fluorescence microscopy with 300 msec exposure times to detect GFP and DsRed, and by differential interference contrast (DIC) microscopy to detect the cells. Scale bar, 2 μm.