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Effects on ER-retained msGFP constructs of including the α-factor pro region after the Ost1 signal sequence. This experiment was performed in parallel with that of Figure 2 with the same parameters, except that msGFP with a C-terminal HDEL signal was fused to either the Ost1 signal sequence alone (pre-Ost1-msGFP*-HDEL) or the Ost1 signal sequence followed by the α-factor pro region (pre-Ost1-pro-αf-msGFP*-HDEL). Scale bar, 2 μm.
