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Effects of the different signal sequences on secretion of msGFP from P. pastoris . Strains of P. pastoris were engineered to express msGFP fused to either the α factor signal sequence alone (pre-αf-msGFP), or the complete α factor secretion signal (pre-pro-αf-msGFP), or the Ost1 signal sequence alone (pre-Ost1-msGFP), or the Ost1 signal sequence followed by the α factor pro region (pre-Ost1-pro-αf-msGFP). Extracellular and intracellular msGFP was analyzed as in Figure 1A.
