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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: J Mol Endocrinol. 2009 Jan 21;42(4):319–330. doi: 10.1677/JME-08-0112

Fig. 4.

Fig. 4

Effects of NGFI-B family members (NGFI-B, NURR-1, NOR-1) on the promoter activity of the genes encoding the enzymes needed for aldosterone synthesis (A, B, and C). H295R cells were co-transfected with each transcription factor (0.1 μg/ml) and each of the steroidogenic enzyme promoter/reporter vectors (1 μg/ml). Identification of NGFIB and SF-1 binding elements in the promoter region of the genes needed for aldosterone synthesis (D). The consensus DNA NGFI-B binding site is distinguished from the SF-1 binding site by 3 base pairs located 5′ to the nuclear receptor half-site: A-A-A for NGFI-B and T/A-C/A-A for SF-1. For analysis of significance, comparison was made with the promoter activity in the presence of the control vector (empty vector = CMV-XL5) shown in gray bars versus promoter activity in the presence of the transcription factor (black bars). Results represent ± S.E.M. for five independent experiments. P<0.05 was considered significant.