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Analysis of Zic3 expression in the mouse embryo. Zic3 expression is visualized in the WT embryo by whole mount in situ hybridization (A) and is not detectable by WISH in Neo embryos (B). Analysis of Zic3 expression using Zic3-LacZ-BAC transgenic mice (C) revealed no expression in the heart grossly (D) or histologically (E). Neo: Zic3NeoIn1/NeoIn1 or Zic3NeoIn1/y. Scale bars in A and B: 0.5 mm.
