Table 3.

Phenotypes of E17.5 Zic3 hypomorphs from six litters

Number of embryos	Overall Phenotype	Cardiac Phenotype
7	Normal (situs solitus)	Normal (levocardia)
1	Exencephaly. Normal otherwise.	Normal (levocardia)
1	Right pulmonary isomerism; asplenia, stomach on left	Mesocardia, TGA, right atrial isomerism, AVSD and symmetrical venous valves
1	Right pulmonary isomerism; asplenia. Stomach on right. Intestines malpositioned outside of liver. Anal atresia	Mesocardia, TGA, right atrial isomerism, AVSD and symmetrical venous valves
1	Right pulmonary isomerism; asplenia. Stomach large and centrally-located.	Mesocardia, TGA, right atrial isomerism, AVSD and symmetrical venous valves
1	Situs ambiguus. Lungs abnormally multi-lobed but not symmetrical.	DORV with VSD. Normal otherwise (levocardia)
1	Situs ambiguus. Lungs abnormally multi-lobed but not symmetrical. Intestinal atresia.	Bottom of heart flat. Single ventricle. Single atrium (right). Only one set of A/V valves. Right descending aorta. Anteriorly-positioned aorta.
1	Situs ambiguus. Lungs abnormally multi-lobed but not symmetrical. Severe exencephaly & anterior neural tube closure defect. Microcephaly and facial malformations (low ears, no eyes).	Mesocardia, large RA, small LA. AVSD. Right descending aorta.
1	Situs ambiguus. Lungs abnormally multi-lobed but not symmetrical. Severe exencephaly & anterior neural tube closure defect. Microcephaly and facial malformations (low-set ears, no eyes). Rib dysplasia.	Large RA, small LA. AVSD. Right descending aorta. TGA.
1	Situs ambiguus. Lungs abnormally multi-lobed but not symmetrical. Severe exencephaly & anterior neural tube closure defect. Microcephaly and facial malformations (low-set ears, no eyes). Rib dysplasia.	AVSD. Right descending aorta. TGA.
1	Pulmonary and abdominal situs inversus.	Dextrocardia. Anteriorly-positioned aorta. DORV with subaortic VSD.
1	Pulmonar and abdominal situs inversus; Microphthalmia.	Dextrocardia. Anteriorly-positioned aorta. DORV with subaortic VSD.