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. 2014 Sep 24;9(9):e108321. doi: 10.1371/journal.pone.0108321

Figure 2. EMSA with −358T or −358C containing oligonucleotides allele and nuclear extracts from HeLa cells.

Figure 2

(A) Nuclear protein extracts from HeLa cells were incubated with biotin-labeled oligonucleotide probes containing −358T or −358C. The figure shows mobility of the labeled oligonucleotides without nuclear extracts (lanes 1 and 7), with nuclear extracts in the absence of competitor (lanes 2 and 8), with nuclear extracts in the presence of various unlabeled competitors as indicated at the bottom autoradiograph (lanes 3–6 and 9–12). The arrow indicates a major oligonucleotide-nuclear protein complex. (B) Nuclear protein extracts from HeLa cells were incubated with biotin-labeled oligonucleotide probes containing −358T or consensus NF-Y binding element. The lower arrow indicates major oligonucleotide-nuclear protein complex without antibody to NF-Y, and the upper arrow shows the protein complex with antibody to NF-Y (lane 5 and 10).