Figure 2. Western blot with corresponding quantification reveals DXR-induced changes in H2AFX phosphorylation were blocked by Bort pre-treatment.

Blot probed with anti-phospho H2AFX antibodies revealed an increase in density of the corresponding 17 kDa band in ovarian lysates harvested 6 hrs post-DXR injection compared to ctl, which is absent in Bort-pretreated samples. *p<0.05, one-way ANOVA, Tukey means comparison. Blot shows β-actin as the loading control. N = 3 blots/quantification.