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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Cell. 2013 Jul 18;154(2):391–402. doi: 10.1016/j.cell.2013.06.010

Figure 4. Dynein-dependent spindle movement drives spindle centering during early anaphase.

Figure 4

(A) Top, time-lapse images showing DHC-GFP in a Type I (left) and Type II (right) cell. Bottom, kymographs showing the movements of dynein at 1 min intervals. Arrow indicates cortical dynein. * indicates spindle movement during early anaphase. (B) Diagram summarizing the 3 types of cell behavior for their anaphase spindle centering processes. Cells were classified based on the position of chromosomes at anaphase onset and the type of membrane elongation. The frequency of each class in control and LGN and 4.1G/R co-depleted cells is indicated. (C) Box plots of Δ (the distance between the cell center and the center of the sister chromatids) in control cells at anaphase onset, and the end of early and late anaphase. The red and blue dashed lines indicate Δ of 1.65, and 0.5, respectively (see Fig. S4A for details). n≥5 cells for each condition. (D) Time-lapse images of equally dividing Type I cells in control (top; Movie S1) and LGN and 4.1G/R co-depleted cells (bottom; Movie S2). (E) Kymographs generated from the image sequences in (D) showing chromosome movements and the cell cortex at 1 min intervals. Scale bars, 10 μm. See also Figure S4.