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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Cell. 2013 Jul 18;154(2):391–402. doi: 10.1016/j.cell.2013.06.010

Figure 5. Asymmetric plasma membrane elongation and cortical dynein coordinately center the spindle during late anaphase.

Figure 5

(A) Merged fluorescent images of symmetrically or asymmetrically elongating cells at the end of early anaphase (magenta) and late anaphase (green) (B) Time-lapse images in Type II and Type III cells (Movie S4, S5). Dashed lines indicate cellular boundaries at early anaphase. (C) Kymographs resulting from image sequences in (B) showing the movements of the chromosomes and the cell cortex at 1 min intervals. (D) Time-lapse images (top) and corresponding kymographs (bottom; 1 min intervals) showing mCherry-H2B and Lifeact-mCherry in unequally dividing Type III cells co-depleted for LGN and 4.1G/R (Movie S6). Dashed lines indicate cellular boundaries at early anaphase. (E) Quantification of membrane elongation in unequally dividing (R>1.5) cells caused by LGN and 4.1G/R co-depletion (n=16). (F) Graph showing sister chromatid distance from the stationary cell cortex for control (black, n=5) and unequally dividing LGN and 4.1G/R co-depleted cells (red, n=5). All sequences were time-aligned with respect to anaphase onset (t=0). Error bars represent the S.E.M. (G) Merged images of cells at the end of early anaphase (magenta) and anaphase B (green) in asymmetrically elongating control (top) and LGN and 4.1G/R co-depleted cells (bottom). (H) Images showing DHC-GFP in asymmetrically elongating control (left) and LGN and 4.1G/R co-depleted cells corresponding to (G). Arrows indicate dynein localization to the stationary cell cortex. Scale bars, 10 μm. See also Figure S5.