Table 1.

Bacterial strains and plasmids used in this study

Strains or plasmids	Relevant characteristics a	Source or reference
Strains
Pseudomonas aeruginosa PA1	Belongs to serum typing group 9 of P. aeruginosa international antigenic typing system; host of phage PaP1	Laboratory collection
P. aeruginosa PA3	Belongs to serum typing group 6 of P. aeruginosa international antigenic typing system; host of phage PaP3	Laboratory collection
E. coli DH5α	Host for the construction of shotgun library clones	Promega, WI, USA
E. coli DH5α cat +:Δnfi	The nfi gene is replaced with cat.	This study
E. coli DH5α Δnfi	The nfi gene is knocked out.	This study
Plasmids
pKD3	Template plasmid for Red system; Ampr, Cmr	[26]
pKD46	Red expression plasmid; Ampr	[26]
pCP20	Flp expression plasmid; Ampr, Cmr	[25]
pUC18	Vector for the construction of shotgun library clones; Ampr	TaKaRa, Shiga, Japan

^aAmp^r, ampicillin resistant; Cm^r, chloramphenicol resistant.