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. 2010 Sep 8;30(36):12138–12150. doi: 10.1523/JNEUROSCI.3245-10.2010

Figure 1.

Figure 1.

Serotonin elicits a different pattern of adult responses in layer V pyramidal neurons after early stress. A, Examples of voltage-clamp traces from control animals show that bath application of 5-HT (10 μm; 30 s) elicited either a dominant outward current (Ai) or no net response (Aii) in layer V pyramidal neurons in the prefrontal cortex. The horizontal line denotes application of 5-HT. The pie chart shows that a large proportion of layer V pyramidal neurons in control animals displayed a 5-HT-induced outward current (Ai; n = 30/35), and a small subset of neurons showed no response (Aii; n = 5/35). B, Examples of voltage-clamp traces from ES animals show that the same application of 5-HT elicited one of three responses: a dominant outward current (Bi), no net response (Bii), or a dominant inward current (Biii) in layer V pyramidal neurons. The dominant inward current was only observed in brain slices from ES animals. The pie chart represents the proportion of layer V pyramidal neurons that displayed outward currents (Bi; n = 27/47), no response (Bii; n = 1/47), or inward currents in response to 5-HT (Biii; n = 19/47) in ES animals. C, Scattergram shows the range of responses in layer V pyramidal neurons to 5-HT in control (n = 35) and ES animals (n = 47). Horizontal line denotes the mean response in layer V pyramidal neurons from control (34.2 ± 3.7 pA) and ES animals (5.9 ± 5.6 pA; *p = 0.0002, unpaired t test).