Figure 3.

Influence of early stress on 5-HT₂ receptor binding and the sensitivity of the 5-HT_2A-elicited inward current in layer V pyramidal neurons. A, ES caused a small increase in [³H]ketanserin binding in the PFC in adulthood. Shown are representative autoradiograms and graphical representation of the 5HT₂ receptor binding density from the PFC of control (C) and ES animals. Data are percentage of control and are mean ± SEM (*p = 0.02, unpaired t test, n = 4 control, n = 7 ES). B, Concentration response curves for the 5-HT_2A-elicited inward current in layer V pyramidal neurons from control and ES animals. These experiments assessed the inward current mediated directly by 5-HT_2A receptors on layer V pyramidal cells by recording after the application of WAY 100635 (30 nm, 10 min) to block 5-HT_1A receptors and in the presence of antagonists to block glutamate and GABA synaptic transmission (CNQX, 20 μm; APV, 50 μm; bicuculline, 10 μm). The EC₅₀ values are not significantly different between groups: (control, 5.6 ± 1.9 μm, n = 5; ES, 5.1 ± 1.9 μm, n = 5; unpaired t test, p = 0.8).