Table 1. Physicochemical and biological properties of waters used for determining the effects of light on rates of nitrification in the absence of rapidly growing phytoplankton.
Station M1 | Station M2 | ||||
30 m | 60 m | 30 m | 60 m | 200 m | |
Pressure | 30.2 | 60.5 | 31.5 | 61.4 | 202 |
NO3−+NO2− (µM) | 12.1 | 18.9 | 5.1 | 19.6 | 30.6 |
Chlorophyll (µg L−1) | 0.8 | 0.1 | 1.7 | B.D.† | B.D. |
PAR (% of Surface) | 1.5% | 0.1% | 6% | 0.1% | <0.1% |
WCA amoA (genes L−1)†† | 4×106 | 4×106 | 1×106 | 6×106 | 6×106 |
WCB amoA (genes L−1) | 9×104 | 2×105 | 9×103 | 3×104 | 4×106 |
AOB amoA (genes L−1) | 2×105 | 3×105 | 9×104 | 5×104 | 2×104 |
WCA amoA (mRNA L−1) | 4×105 | 3×105 | 6×104 | 2×106 | 1×106 |
WCB amoA (mRNA L−1) | 2×103 | 5×103 | B.D.† | 3×104 | 3×105 |
AOB amoA (mRNA L−1) | 8×104 | 2×104 | 6×103 | 5×104 | B.D. |
B.D, below detection (<0.01 µg L−1 chlorophyll or <10 copies per qPCR reaction for AOB amoA).
Gene and transcript abundances obtained by qPCR were normalized to the volume of seawater filtered.