Table 3. Quantitative analysis of DNA purity using VCQA employing qPCR.
Genes | E | pMD18-T-VCQA | DH366 | ΔCT(control-sample) | (1+E)ΔCT(control-sample) | ccmB/actin | rpoB/actin |
actin | 1.124 | 17.79 | 23.70 | −5.91 | 0.0117 | – | – |
ccmB | 1.060 | 17.64 | 17.87 | −0.23 | 0.8469 | 72 | – |
rpoB | 1.042 | 17.76 | 13.40 | 4.36 | 22.4824 | – | 1922 |
VCQA = Vector Control Quantitative Analysis; E = efficiency of amplification; CT = number of cycles.