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. 2014 Sep 9;14:334. doi: 10.1186/1472-6882-14-334

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© Engel et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Apoptosis determination via Annexin V/PI staining. A: Histogramms of Annexin V/PI- stained MCF-7 cells after treatment with 10 μg/ml of the four plant extracts in comparison with the control treatment for 48 h. Annexin V in conjunction with PI staining was used to distinguish early apoptotic (Annexin V- positive, PI negative; quadrant 1 of each panel) from late apoptotic or necrotic cells (Annexin V positive, PI positive; quadrant 2 of each panel). B: Table of quantitative analysis of single Annexin V or PI positive and double positive stained MCF-7 cells. Notably, sub-G1 phase positive cells from the cell cycle measurement were listed for comparison. Results are representative of three separate experiments.