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. 2014 Sep 9;14:334. doi: 10.1186/1472-6882-14-334

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© Engel et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Protein expression analysis of MCF-7 cells by Western blotting after treatment with 10 μg/ml of the four plant extracts in comparison with the control treatment for 48 h. A: Determination of caspase 7, 8, and 9 cleavage. Detected were the pro-caspases as well as the cleaved proteins. B: Expression of total focal adhesion kinase (FAK) protein and phosphorylated FAK protein at residue Tyr397. C: Protein content of kinases: total Akt, phosphorylated Akt at residue S473 (pAkt), total MAPK p44/42, phosphorylated MAPK p44/42 at residues T202/204 (P-p44/42) and β-Actin which did not function as housekeeping protein. D: Stain-free image of separated proteins after SDS-PAGE to ensure equal protein amounts on each polyacrylamide gel used.